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Functional analysis of the plant disease resistance gene ...

Pto is a serine/threonine kinase that mediates resistance in tomato to strains of Pseudomonas syringae pv. tomato expressing the (a)virulence proteins AvrPto or AvrPtoB. DNA shuffling was used as a combinatorial in vitro genetic approach to dissect the functional regions of Pto.

Two Distinct Pseudomonas Effector Proteins Interact with ...

The Pto serine/threonine kinase of tomato confers resistance to speck disease by recognizing strains of Pseudomonas syringae that express the protein AvrPto. Pto and AvrPto physically interact, and this interaction is required for activation of host resistance. We identified a second Pseudomonas protein, AvrPtoB, that interacts specifically with Pto and is widely distributed among plant pathogens.

Effector-triggered immunity mediated by the Pto kinase ...

 · Pto was the first disease-resistance gene cloned from a plant that confers recognition of a specific pathogen. The intracellular protein kinase that it encodes activates an immune response in tomato (Solanum lycopersicum) to bacterial speck disease by interacting with either the AvrPto or AvrPtoB type III effector proteins that are delivered into the plant cell by Pseudomonas syringae …

AvrPtoB: A bacterial type III effector that both elicits ...

immunity when inoculated on tomato plants carrying the Pto R protein. Pto is a serine/threonine protein ki-nase that elicits PCD and immunity when it interacts with the DC3000 effector proteins AvrPto or AvrPtoB [10]. Pto also requires a second protein, Prf, to signal HR-based PCD [11]. On tomato plants lacking Pto,

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Overexpression of Pti4, Pti5, and Pti6 in tomato promote ...

 · Pseudomonas syringae pv.tomato (Pst) is a pathogenic microorganism that causes bacterial speck disease and affects tomato yield and quality.Pto is a disease resistant gene for plant to recognize and defense against Pst.Pto interacts with Pti (Pto interacting) proteins, which include three transcription factors, Pti4, Pti5, Pti6, and they were thought to be downstream of Pto-mediated …

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The Plant Journal The N-terminal region of Pseudomonas ...

The N-terminal region of Pseudomonas type III effector AvrPtoB elicits Pto-dependent immunity and has two distinct virulence determinants Fangming Xiao1, Ping He2, Robert B. Abramovitch1,3, Jennifer E. Dawson4, Linda K. Nicholson4, Jen Sheen2 and Gregory B. Martin1,3,* 1Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA, 2Department of Molecular Biology ...

Initiation of Plant Disease Resistance by Physical ...

Resistance to bacterial speck disease in tomato occurs when the Pto kinase in the plant responds to expression of the avirulence gene avrPto in the Pseudomonas pathogen. Transient expression of an avrPto transgene in plant cells containing Pto elicited a defense response. In the yeast two-hybrid system, the Pto kinase physically interacted with AvrPto.

Can we require an employee to use paid time off (PTO) if ...

When there are no legal requirements, such as state and local paid sick leave laws, restrictions on the amount of notice required and the increments in which PTO may be used, are common.

Expression of the Tomato Pto Gene in Tobacco ...

The Pto-transformed tobacco plants exhibited a significant increase in resistance to the avirulent P. s. tabaci strain compared with wild-type tobacco as indicated by (1) more rapid development of a hypersensitive resistance response at high inoculum concentrations (108 colony-forming units per mL); (2) lessened severity of disease symptoms at ...

The T-loop Extension of the Tomato Protein Kinase AvrPto ...

 · Another gene that was identified as a Pto-interacting protein was the tomato protein kinase Adi3, which only interacts with Pto in the presence of AvrPto . Subsequently, we have shown Adi3 to function as a negative regulator of plant cell death ( 13 ) and thus, it may be the functional homologue of PKB (aka Akt), a major PCD suppressor in ...

Plant Trait Ontology | NCBO BioPortal

Acronym. PTO. Visibility. Public. Description. A controlled vocabulary to describe phenotypic traits in plants. Each trait is a distinguishable feature, characteristic, quality or phenotypic feature of a developing or mature plant, or a plant part. Status. Production.

PTO and work life balance at Plant Partners, Inc | Indeed

 · How to Calculate PTO When an employer offers you a full-time job, they often include a benefits package. This package typically includes healthcare, dental, vision, retirement plans and PTO. PTO is an important benefit for employers to offer because it gives employees the chance to decompress, stay at home while their sick or take care of errands while still getting paid.

Plants expressing the Pto disease resistance gene confer ...

 · Pto‐expressing plants were resistant to viral infection while untransformed plants developed local lesions on the inoculated leaves 4–5 days post‐inoculation ( Fig. 3a). These lesions grew to approximately 1 cm in diameter and within 1 week cell death appeared in upper, uninoculated leaves. Susceptible plants eventually died.

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Genetic characterization of the Pto locus of tomato: semi ...

Genetic characterization of the Pto locus of tomato: semi-dominance and cosegregation of resistance to Pseudomonas syringae pathovar tomato and sensitivity to the insecticide Fenthion. Carland FM(1), Staskawicz BJ. Author information: (1)Department of Plant Pathology, University of …

Plants expressing the Pto disease resistance gene confer ...

Plants expressing the Pto disease resistance gene confer resistance to recombinant PVX containing the avirulence gene AvrPto Plant J. 1999 Jan;17(1):41-50. doi: 10.1046/j.1365-313x.1999.00350.x. Authors C M Tobias 1, G E Oldroyd, J H Chang, B J Staskawicz. Affiliation 1 Department of ...

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Pto DC3000

Systematic characterizations of these interactions is being modeled using Gene Ontology (GO) Annotation. Virulence factors with associated GO annotation. View interaction diagram. Download, sort, and print annotations in Excel. Key to modified Gene Ontology Annotation used to describe the Pto DC3000-host interaction. Frequently Asked Questions.

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PTO and work life balance at Morton Plant Hospital ...

Guide to Understanding How PTO Works Paid time off (PTO) is a benefit an employer provides their employees that allows them to receive payment for the time they take off work for vacation, personal days, sick time and holidays. A company''Parent benefits''s PTO policy works, you need to know the amount of paid time off your employer provides you, how you earn or accrue that time and the rules ...

AvrPto-dependent Pto-interacting proteins and AvrPto ...

 · The plant-intracellular interaction of the avirulence protein AvrPto of Pseudomonas syringae pathovar tomato, the agent of bacterial speck disease, and the corresponding tomato resistance protein Pto triggers responses leading to disease resistance. Pto, a serine/threonine protein kinase, also interacts with a putative downstream kinase, Pto-interactor 1, as well as with members of a family of ...

Overexpression of the Disease Resistance Gene Pto in ...

The Pto gene encodes a serine/threonine protein kinase that confers resistance in tomato ( Lycopersicon esculentum ) to Pseudomonas syringae pv tomato strains that express the type III effector protein AvrPto. Constitutive overexpression of Pto in tomato, in the absence of AvrPto, activates defense responses and confers resistance to several diverse bacterial and fungal plant pathogens.

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CHESS News 2007

 · Contact: D. M. Szebenyi ([email protected] ) Plant pathogens use "effector proteins" to enhance the infection process in host plants. Plants, in turn, have evolved a defense mechanism in which a pathogen''s effector is recognized by a corresponding plant disease resistance protein, and a "hypersensitive response" is triggered, leading to rapid localized cell death which limits the spread of …

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